| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.057903 |
| Chromosome: | chromosome 1 |
| Location: | 5741591 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g041000 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATTCCTTACGCCTGTCACACTTCCAGTTAT |
| Internal bar code: | TTGTGATTCCTCAGGGCGCGCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 305 |
| LEAP-Seq percent confirming: | 99.6334 |
| LEAP-Seq n confirming: | 3261 |
| LEAP-Seq n nonconfirming: | 12 |
| LEAP-Seq n unique pos: | 16 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCGTCATGTTCGCTTCTACG |
| Suggested primer 2: | CGATGTGTCTCAAAGGCTCA |