| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.057925 |
| Chromosome: | chromosome_8 |
| Location: | 3419943 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre08.g374700 | FAP244 | Flagellar Associated Protein | sense | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TGTTTGGAAATGCGGTACAGGAGGGGGGGG |
| Internal bar code: | GGACAGTACGGGAAAACGGCGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 829 |
| LEAP-Seq percent confirming: | 44.3998 |
| LEAP-Seq n confirming: | 1106 |
| LEAP-Seq n nonconfirming: | 1385 |
| LEAP-Seq n unique pos: | 14 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCCGTTGCAAGCTGTTTTAT |
| Suggested primer 2: | GCAAGGCGAAGTGAAGAAAC |