| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.057928 |
| Chromosome: | chromosome 7 |
| Location: | 1371324 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g322500 | MRPL19 | Mitochondrial ribosomal protein L19 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AAGACAGCTGACAGAGCGACGGGGTCCTTC |
| Internal bar code: | AAAACGTGTAGCCCGGTCATAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 252 |
| LEAP-Seq percent confirming: | 97.8166 |
| LEAP-Seq n confirming: | 448 |
| LEAP-Seq n nonconfirming: | 10 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCAGGACAAGGTATGGTGCT |
| Suggested primer 2: | CCTTTTATGTCCGCACCTGT |