Insertion junction: LMJ.RY0402.057935_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.057935
Chromosome:chromosome 16
Location:7484137
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre16.g687182 POB10 Proteome of basal body 10; (1 of 19) IPR000225//IPR016024 - Armadillo // Armadillo-type fold 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ATTCAGCTCAGGTCGTACGGCTGCGATGGA
Internal bar code:CTGAGGCGGCCAACCAGGCTC

Confirmation - LEAP-Seq

LEAP-Seq distance:976
LEAP-Seq percent confirming:99.7002
LEAP-Seq n confirming:3658
LEAP-Seq n nonconfirming:11
LEAP-Seq n unique pos:4

Suggested primers for confirmation by PCR

Suggested primer 1:CTCACAGCTTATGATCCGCA
Suggested primer 2:GCTTACTTGTGAGGGCTTGC