| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.057939 |
| Chromosome: | chromosome 17 |
| Location: | 3665554 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g726650 | CGL96 | (1 of 1) K14839 - nucleolar protein 16 (NOP16); Conserved in the Green Lineage | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGGGTAGGTGCACACGTGCAGGTGTGAGTA |
| Internal bar code: | GGGGCAGATCAAGCGGATGAAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 872 |
| LEAP-Seq percent confirming: | 97.3963 |
| LEAP-Seq n confirming: | 3404 |
| LEAP-Seq n nonconfirming: | 91 |
| LEAP-Seq n unique pos: | 26 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGACCAGAGAACGCAGTGTC |
| Suggested primer 2: | CCCAACTCCAAACACGACTT |