| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.057974 |
| Chromosome: | chromosome 10 |
| Location: | 1258790 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g426850 | (1 of 3) PF02458 - Transferase family (Transferase) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGCGGCCTGGGGCATGCCTATGACTTCCC |
| Internal bar code: | GACGTTCGTTTCCGATCTGAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1036 |
| LEAP-Seq percent confirming: | 99.6838 |
| LEAP-Seq n confirming: | 8828 |
| LEAP-Seq n nonconfirming: | 28 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGATGGTGAGAGTTTGAGCG |
| Suggested primer 2: | CCAACATATAGCTCTCGGGC |