| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.057975 |
| Chromosome: | chromosome 14 |
| Location: | 257752 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g609400 | (1 of 6) 1.8.4.11 - Peptide-methionine (S)-S-oxide reductase / Peptide methionine sulfoxide reductase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCCAGTTCCCTCATCAGGTCTTTGGAACAC |
| Internal bar code: | AGCTTACACCTGAAACGTGCGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 266 |
| LEAP-Seq percent confirming: | 99.7946 |
| LEAP-Seq n confirming: | 8261 |
| LEAP-Seq n nonconfirming: | 17 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AACGGACTGGGGAAGAAGTT |
| Suggested primer 2: | ACTGAGACCACCCCTGAATG |