| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.057975 |
| Chromosome: | chromosome 14 |
| Location: | 1064642 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g614750 | CPNAT1,cpNAT1,NAT34 | (1 of 1) 2.3.1.189 - Mycothiol synthase; Ribosome-associated chloroplast-localized N-acetyltransferase | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCCCCGCGCGTGAAACGTGATTTGGGCAGG |
| Internal bar code: | GGATAATGTGAATAAATGAGGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 928 |
| LEAP-Seq percent confirming: | 99.246 |
| LEAP-Seq n confirming: | 2106 |
| LEAP-Seq n nonconfirming: | 16 |
| LEAP-Seq n unique pos: | 23 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTCACGGCAAACCATGTAT |
| Suggested primer 2: | TTCATTCTCCCCTCACATCC |