| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.057988 |
| Chromosome: | chromosome 16 |
| Location: | 80255 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| intergenic |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATACATGGGGCCGAAATTGATTTTCTTACC |
| Internal bar code: | ATCCACTGTCGCCATCGTAGAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 802 |
| LEAP-Seq percent confirming: | 99.6295 |
| LEAP-Seq n confirming: | 3496 |
| LEAP-Seq n nonconfirming: | 13 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGTATGTGTGTCTTGGTGCC |
| Suggested primer 2: | ATGGCGAATTGGTAAGCATC |