| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.058000 |
| Chromosome: | chromosome 7 |
| Location: | 1688022 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g325550 | KDG3 | Diacylglycerol kinase; (1 of 2) K00901 - diacylglycerol kinase (ATP) (dgkA, DGK) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGCGAGTCGTGCTCCATGTACGTGCATATG |
| Internal bar code: | TTCTCAGGGTCCCGTTCGCGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 803 |
| LEAP-Seq percent confirming: | 93.9024 |
| LEAP-Seq n confirming: | 308 |
| LEAP-Seq n nonconfirming: | 20 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGAGCCACCTTTCTTGTCG |
| Suggested primer 2: | GGAACATCACTGTCACCACG |