| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.058002 |
| Chromosome: | chromosome 1 |
| Location: | 3056055 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g018850 | EEP2 | (1 of 4) K12603 - CCR4-NOT transcription complex subunit 6 (CNOT6, CCR4); Exonuclease-Endonuclease-Phosphatase superfamily protein | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTGGGACCCCCGCGCTGCATCCCCGCCCG |
| Internal bar code: | TAGCGGTGTAGATGGCTTTGAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 152 |
| LEAP-Seq percent confirming: | 80.8303 |
| LEAP-Seq n confirming: | 662 |
| LEAP-Seq n nonconfirming: | 157 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAAAGCAGACACATGCAGGA |
| Suggested primer 2: | CGCTTGCGGTATTACGGTAT |