| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.058006 |
| Chromosome: | chromosome 10 |
| Location: | 5533700 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g459450 | SUB10 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGTTCGAGGCTTTTGCGAGCCATACAGGAA |
| Internal bar code: | CGCTATTTGCTTTTGCTGTAAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 565 |
| LEAP-Seq percent confirming: | 99.6816 |
| LEAP-Seq n confirming: | 6887 |
| LEAP-Seq n nonconfirming: | 22 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GACGTCTAACACCGGCAAAT |
| Suggested primer 2: | TCTGACCATGGAGCAGAGC |