| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.058006 |
| Chromosome: | chromosome 10 |
| Location: | 5533727 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g459450 | SUB10 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCCGAGCCATTGCATGGTGGTGGCCCGCA |
| Internal bar code: | TAGTGGCGGACCGCGACGGAAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 237 |
| LEAP-Seq percent confirming: | 25.9181 |
| LEAP-Seq n confirming: | 367 |
| LEAP-Seq n nonconfirming: | 1049 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GACGTCTAACACCGGCAAAT |
| Suggested primer 2: | TCTGACCATGGAGCAGAGC |