| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.058014 |
| Chromosome: | chromosome_12 |
| Location: | 4395716 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre12.g520650 | TLP1 | Tubby-like protein | antisense | CDS |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TCTTTGCAGACCCGCGGTCGCGCACGATGA |
| Internal bar code: | GCGAGAGCCAAAAGGGGGGGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1012 |
| LEAP-Seq percent confirming: | 99.5023 |
| LEAP-Seq n confirming: | 6398 |
| LEAP-Seq n nonconfirming: | 32 |
| LEAP-Seq n unique pos: | 57 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGCTGGAAGTTCTTCACGGA |
| Suggested primer 2: | GTGTGCTGCTTGGGGTAAAT |