Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | - |
Strain: | LMJ.RY0402.058014 |
Chromosome: | chromosome_12 |
Location: | 7136482 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre12.g559050 | antisense | intron |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | TGCAAAATCCTGCCTGCGGTCCCCCAGGTT |
Internal bar code: | CGCCGGGATACTTGCCCCGTC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 718 |
LEAP-Seq percent confirming: | 65.942 |
LEAP-Seq n confirming: | 91 |
LEAP-Seq n nonconfirming: | 47 |
LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | TCGATTGCTTGAATGTGAGC |
Suggested primer 2: | AGTTGGACGCCTGCTGTAGT |