Insertion junction: LMJ.RY0402.058017_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.058017
Chromosome:chromosome 3
Location:1724768
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre03.g153550 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACACCCACACCCACCCACGCGCGTGTTTGC
Internal bar code:ACTTGGCGCCTCTGGGAAGGGC

Confirmation - LEAP-Seq

LEAP-Seq distance:544
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:338
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:CATACAAATCCCACCCCATC
Suggested primer 2:GCACCGACCTCATCCATACT