| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.058020 |
| Chromosome: | chromosome 2 |
| Location: | 3455305 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g095116 | (1 of 781) IPR000104 - Antifreeze protein, type I | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGGATCGGGCTGCGGGTCGAGATGCGGGAA |
| Internal bar code: | CTGGCGTATGGGGGTACAGTCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 453 |
| LEAP-Seq percent confirming: | 82.6259 |
| LEAP-Seq n confirming: | 1460 |
| LEAP-Seq n nonconfirming: | 307 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACTCTCTCCAGGGGCTATG |
| Suggested primer 2: | GGTTTGCCTGTAGCCACG |