Insertion junction: LMJ.RY0402.058030_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.058030
Chromosome:chromosome 12
Location:7884644
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre12.g552750 (1 of 2) PF04520 - Senescence regulator (Senescence_reg) 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTGGAACCGAAGTCGGGGCTGTCCGAGCTG
Internal bar code:TACACAGTGTTGATATCAGATG

Confirmation - LEAP-Seq

LEAP-Seq distance:988
LEAP-Seq percent confirming:99.3488
LEAP-Seq n confirming:9916
LEAP-Seq n nonconfirming:65
LEAP-Seq n unique pos:18

Suggested primers for confirmation by PCR

Suggested primer 1:ACACACACACACACACACCG
Suggested primer 2:GCTAGACTTGGCCGATGAAG