| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.058042 |
| Chromosome: | chromosome 10 |
| Location: | 2394911 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g435300 | AAP1 | Aspartyl aminopeptidase-like protein; (1 of 1) 3.4.11.21 - Aspartyl aminopeptidase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTTCATGGACGTGGCCCTATTGGCGATGAC |
| Internal bar code: | GTATGCGTCAACCCTCGGCCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 532 |
| LEAP-Seq percent confirming: | 99.366 |
| LEAP-Seq n confirming: | 5799 |
| LEAP-Seq n nonconfirming: | 37 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTTTGGAGCAAGCAGAGGTT |
| Suggested primer 2: | GTCTCAACTTCTTGGCTCGG |