Insertion junction: LMJ.RY0402.058042_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.058042
Chromosome:chromosome 10
Location:2394925
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre10.g435300 AAP1 Aspartyl aminopeptidase-like protein; (1 of 1) 3.4.11.21 - Aspartyl aminopeptidase 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTTACTCGAGCATGTGGGTCTGTGAGGTCT
Internal bar code:TTTTCCGCGAAAGCGCAATCAC

Confirmation - LEAP-Seq

LEAP-Seq distance:814
LEAP-Seq percent confirming:83.9611
LEAP-Seq n confirming:691
LEAP-Seq n nonconfirming:132
LEAP-Seq n unique pos:14

Suggested primers for confirmation by PCR

Suggested primer 1:TTTTGGAGCAAGCAGAGGTT
Suggested primer 2:GTCTCAACTTCTTGGCTCGG