| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.058045 |
| Chromosome: | chromosome 10 |
| Location: | 2619454 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g437300 | (1 of 1) PF00053 - Laminin EGF domain (Laminin_EGF) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCTGCCAGTTGCCGCTGCTTGTGCAGCAGG |
| Internal bar code: | GGTGAACGTAGGCTAGGGGCAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 961 |
| LEAP-Seq percent confirming: | 99.4086 |
| LEAP-Seq n confirming: | 5883 |
| LEAP-Seq n nonconfirming: | 35 |
| LEAP-Seq n unique pos: | 24 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGCTACTCACTCAGCCGTG |
| Suggested primer 2: | GAACGTCTGGGAGCTTTACG |