| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.058054 |
| Chromosome: | chromosome 2 |
| Location: | 3996301 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g096950 | (1 of 1) PF09444 - MRC1-like domain (MRC1) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCGGATCCAGCCGCCGCCAGCGACACCTGC |
| Internal bar code: | CACGCGTATCTCATGCACGCCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 668 |
| LEAP-Seq percent confirming: | 97.2968 |
| LEAP-Seq n confirming: | 5831 |
| LEAP-Seq n nonconfirming: | 162 |
| LEAP-Seq n unique pos: | 41 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AACAAAACGAAGCCCGTATG |
| Suggested primer 2: | TTGAGTTGAACAACGGAACG |