Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | + |
Strain: | LMJ.RY0402.058058 |
Chromosome: | chromosome 3 |
Location: | 6297372 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre03.g193200 | (1 of 781) IPR000104 - Antifreeze protein, type I | CDS |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGGAGGAGACGGCCGCGGCTGCGTGTGCCT |
Internal bar code: | GATTTGAGTGAACTGCCTCGAC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 1128 |
LEAP-Seq percent confirming: | 99.6833 |
LEAP-Seq n confirming: | 20460 |
LEAP-Seq n nonconfirming: | 65 |
LEAP-Seq n unique pos: | 24 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CCTCGTACTCCTCTTGCGTC |
Suggested primer 2: | GGAGAACCTGAGGGTGCATA |