| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.058062 |
| Chromosome: | chromosome 2 |
| Location: | 5198138 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g105750 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCCCCGGCCGCAGGCGGTTCGCTCTTCCCC |
| Internal bar code: | ACACTGCGATTTGGACGCAGTCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 528 |
| LEAP-Seq percent confirming: | 97.7209 |
| LEAP-Seq n confirming: | 2444 |
| LEAP-Seq n nonconfirming: | 57 |
| LEAP-Seq n unique pos: | 31 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGAAGTGCACGTAAAGCCAG |
| Suggested primer 2: | CAGCTAGCGGTAGTGACACG |