| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.058066 |
| Chromosome: | chromosome 6 |
| Location: | 1229287 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g257750 | (1 of 2) IPR023150 - Double Clp-N motif | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAAAGTTGCGGCTCGCAGTGGCTGGCTGGG |
| Internal bar code: | GTTTGCCGTTTGGCCGCGGGCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 638 |
| LEAP-Seq percent confirming: | 99.3994 |
| LEAP-Seq n confirming: | 331 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGCTGTCCAGGTCTAGGGAC |
| Suggested primer 2: | CTGGTGTGTCCTTGAACCCT |