| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.058122 |
| Chromosome: | chromosome 6 |
| Location: | 4914303 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g279350 | (1 of 1) 3.5.1.17 - Acyl-lysine deacylase / Epsilon-lysine acylase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAAGCACGCAATCATGGACAAGAGGAACAG |
| Internal bar code: | CCCGCGGTTTAACTGTATTAAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 682 |
| LEAP-Seq percent confirming: | 99.1367 |
| LEAP-Seq n confirming: | 3445 |
| LEAP-Seq n nonconfirming: | 30 |
| LEAP-Seq n unique pos: | 20 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTTGAGCTTGAAGTAGCCCG |
| Suggested primer 2: | CATAGAGCTGGACCTGAGCC |