| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.058123 |
| Chromosome: | chromosome 2 |
| Location: | 2222163 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g089850 | (1 of 1) IPR000969//IPR013719 - Structure-specific recognition protein // Domain of unknown function DUF1747 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCTTCCTCGCGGCCAGCGCCTGCCCCCGAC |
| Internal bar code: | AGAGCGCGGTATGGAGCCTGAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1250 |
| LEAP-Seq percent confirming: | 99.6155 |
| LEAP-Seq n confirming: | 7514 |
| LEAP-Seq n nonconfirming: | 29 |
| LEAP-Seq n unique pos: | 16 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACATTTGACCCAGCATGTC |
| Suggested primer 2: | TCCCCTCTCCTGTCTTCTCA |