Insertion junction: LMJ.RY0402.058131_3

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.058131
Chromosome:chromosome 11
Location:568413
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre11.g467608 (1 of 4) IPR003882//IPR008752 - Pistil-specific extensin-like protein // Peptidase M11, gametolysin CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCTACATCGAGCCGGGCGTGGCGGCCGGGC
Internal bar code:GATAAAGGAAACCGTTGACTGC

Confirmation - LEAP-Seq

LEAP-Seq distance:101
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:210
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:ATGGGACAGGGTGGTAGTCA
Suggested primer 2:CGCAGCTACACCACATGAAC