| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.058133 |
| Chromosome: | chromosome_3 |
| Location: | 4257105 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre03.g174250 | FAP111 | Flagellar Associated Protein | antisense | CDS |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | CCTGCCGCAAGCTGCTTTTGGTCCTGGAGT |
| Internal bar code: | ATGGGTAGTTGCCATGGACCCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 875 |
| LEAP-Seq percent confirming: | 99.4463 |
| LEAP-Seq n confirming: | 898 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTTCAGGCTCATCAGGTGGT |
| Suggested primer 2: | ATGGGAATGAGCATTTCTGC |