| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.058224 |
| Chromosome: | chromosome 3 |
| Location: | 5758576 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g188450 | PTX2 | Phototaxis regulator | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGAACGAGCACTGCATACGATACTGCCGAT |
| Internal bar code: | TTAGATTTCTAACAATTCCCCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1263 |
| LEAP-Seq percent confirming: | 99.6993 |
| LEAP-Seq n confirming: | 12929 |
| LEAP-Seq n nonconfirming: | 39 |
| LEAP-Seq n unique pos: | 15 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCAACATGCACTTCCAGCTC |
| Suggested primer 2: | CATTGTCTGGTGTGTGAGGG |