| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.058245 |
| Chromosome: | chromosome 5 |
| Location: | 679529 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g230800 | (1 of 1) PF00176//PF07496 - SNF2 family N-terminal domain (SNF2_N) // CW-type Zinc Finger (zf-CW) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAACCCACGAGAAGTATGCGCCCCCGCCGC |
| Internal bar code: | AATTATTGTGCGGATGTTTCTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 348 |
| LEAP-Seq percent confirming: | 99.5822 |
| LEAP-Seq n confirming: | 22884 |
| LEAP-Seq n nonconfirming: | 96 |
| LEAP-Seq n unique pos: | 18 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCATATAGCTTGAAAGCGGC |
| Suggested primer 2: | CTGATGAGGAGCAGGAGGAG |