| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.058246 |
| Chromosome: | chromosome 9 |
| Location: | 2255665 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g392650 | MOT39 | Predicted protein; (1 of 1) PTHR14991//PTHR14991:SF0 - FAMILY NOT NAMED // RING FINGER PROTEIN 32 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGGGCTGGTATCTACAACCCCTCCCTACT |
| Internal bar code: | ATGTGTCGAGCTATAGCTCATA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 159 |
| LEAP-Seq percent confirming: | 66.0714 |
| LEAP-Seq n confirming: | 37 |
| LEAP-Seq n nonconfirming: | 19 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTTTCGTTGCAGTAGGGAG |
| Suggested primer 2: | TTTCGCACTGAACTCGAATG |