| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.058249 |
| Chromosome: | chromosome 8 |
| Location: | 4944424 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g385400 | (1 of 3) 3.5.1.1 - Asparaginase / L-asparagine amidohydrolase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACCAGGTGCATGGCGCGGCCCTGACCTGCA |
| Internal bar code: | CAAAAACCAGGCCCACCCACCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 675 |
| LEAP-Seq percent confirming: | 99.604 |
| LEAP-Seq n confirming: | 4527 |
| LEAP-Seq n nonconfirming: | 18 |
| LEAP-Seq n unique pos: | 18 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGCGACTGGAAGGAGACTTT |
| Suggested primer 2: | GATTTGCTCAGTGTCGGGTT |