| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.058253 |
| Chromosome: | chromosome 3 |
| Location: | 3842953 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g170700 | (1 of 3) 3.2.1.39 - Glucan endo-1,3-beta-D-glucosidase / Laminarinase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAGTGTGTCGACTGGGTTTTGCTGGTGTGC |
| Internal bar code: | GGCGGGTACGAAAGCGTCGCTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 536 |
| LEAP-Seq percent confirming: | 99.5526 |
| LEAP-Seq n confirming: | 1335 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACACGTGGCAGTGCTTGTAG |
| Suggested primer 2: | GTGCCAAGTAAACCGTCGAT |