| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.058293 |
| Chromosome: | chromosome 16 |
| Location: | 5878971 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g677450 | (1 of 2) 5.1.3.15 - Glucose-6-phosphate 1-epimerase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATCGCGGGTCTGCGCCGAGGACCGGACCGC |
| Internal bar code: | TCCTGGCCCCGTCCTTGGGTTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1273 |
| LEAP-Seq percent confirming: | 98.5939 |
| LEAP-Seq n confirming: | 7713 |
| LEAP-Seq n nonconfirming: | 110 |
| LEAP-Seq n unique pos: | 15 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACCCTCACCTGACCATGAAG |
| Suggested primer 2: | GCGACCCAGTTTCATGTTTT |