Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.058294 |
Chromosome: | chromosome 10 |
Location: | 163078 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre10.g418900 | (1 of 18) K13412 - calcium-dependent protein kinase (CPK) | 3'UTR |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGCTTTGTAGCAGCGAGAAACCACTTGCA |
Internal bar code: | GGACAGCGTTGAGCATGGATGC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 281 |
LEAP-Seq percent confirming: | 98.972 |
LEAP-Seq n confirming: | 9724 |
LEAP-Seq n nonconfirming: | 101 |
LEAP-Seq n unique pos: | 30 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | ACGAGTACGGTATTCACGCC |
Suggested primer 2: | CTGGACCTCTCGGACCAGT |