| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.058294 |
| Chromosome: | chromosome 12 |
| Location: | 5253472 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g528350 | (1 of 1) IPR000408//IPR001810//IPR009091 - Regulator of chromosome condensation, RCC1 // F-box domain // Regulator of chromosome condensation 1/beta-lactamase-inhibitor protein II | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCAGCGTCTCACAGACTCCCACCTGGCAG |
| Internal bar code: | TTAGGGCGCTTTAACAGAAGGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1411 |
| LEAP-Seq percent confirming: | 99.6848 |
| LEAP-Seq n confirming: | 17712 |
| LEAP-Seq n nonconfirming: | 56 |
| LEAP-Seq n unique pos: | 16 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCAGGCAGTCTTGACCACAG |
| Suggested primer 2: | GACCCAGACCCTAACGACAA |