| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.058315 |
| Chromosome: | chromosome 3 |
| Location: | 5106699 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g181500 | DPE1,STA11 | Disproportionating enzyme 1; (1 of 1) PTHR32438:SF5 - 4-ALPHA-GLUCANOTRANSFERASE DPE1, CHLOROPLASTIC/AMYLOPLASTIC | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGTTTGTTGCTTTCTGCTGGTCTCTGCACC |
| Internal bar code: | GTGGAGTCAGGGAAGGCGGAGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 654 |
| LEAP-Seq percent confirming: | 96.4856 |
| LEAP-Seq n confirming: | 1208 |
| LEAP-Seq n nonconfirming: | 44 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TAGGTGTAGGGGTGCGTTTC |
| Suggested primer 2: | TGTACAGAAATGCAACCCGA |