Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.058324 |
Chromosome: | chromosome_11 |
Location: | 327149 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre11.g467567 | UBQ1 | Ubiquitin | antisense | 3'UTR |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | CAAAACGCCCACAGCCAACGTCCTTCAGCC |
Internal bar code: | TTTCGAGTAGCACAAGCTGGAA |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 685 |
LEAP-Seq percent confirming: | 98.8571 |
LEAP-Seq n confirming: | 2249 |
LEAP-Seq n nonconfirming: | 26 |
LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CCCAGCTTGACGAAAAGTTC |
Suggested primer 2: | ACCATTGAGAACGTGAAGGC |