| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.058343 |
| Chromosome: | chromosome 1 |
| Location: | 4615127 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g031750 | (1 of 1) K11883 - RNA-binding protein NOB1 (NOB1) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCTGATCGCAGGAAGGACTGCCATGTCCGT |
| Internal bar code: | TGCGGCAACCTTAAAACCTGCCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 717 |
| LEAP-Seq percent confirming: | 99.3499 |
| LEAP-Seq n confirming: | 9017 |
| LEAP-Seq n nonconfirming: | 59 |
| LEAP-Seq n unique pos: | 48 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAACGTGGGAGGGCTAAGTT |
| Suggested primer 2: | CCGGCATAGCATGTAGGAAT |