Insertion junction: LMJ.RY0402.058348_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.058348
Chromosome:chromosome 2
Location:6959467
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre02.g120301 SELH,SELENOH,SELH1 (1 of 133) IPR012336 - Thioredoxin-like fold; Selenoprotein H 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACTGCGGGCGATTGGGAGGAAAGCGCACGG
Internal bar code:CGCGAGGCGATCCATTCGTCAC

Confirmation - LEAP-Seq

LEAP-Seq distance:1126
LEAP-Seq percent confirming:99.3547
LEAP-Seq n confirming:3387
LEAP-Seq n nonconfirming:22
LEAP-Seq n unique pos:7

Suggested primers for confirmation by PCR

Suggested primer 1:CCTCCGCACATAGACCATTT
Suggested primer 2:GGGTTGTAGATACCAGCCCA