Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | + |
Strain: | LMJ.RY0402.058348 |
Chromosome: | chromosome 2 |
Location: | 6959467 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre02.g120301 | SELH,SELENOH,SELH1 | (1 of 133) IPR012336 - Thioredoxin-like fold; Selenoprotein H | 3'UTR |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACTGCGGGCGATTGGGAGGAAAGCGCACGG |
Internal bar code: | CGCGAGGCGATCCATTCGTCAC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 1126 |
LEAP-Seq percent confirming: | 99.3547 |
LEAP-Seq n confirming: | 3387 |
LEAP-Seq n nonconfirming: | 22 |
LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CCTCCGCACATAGACCATTT |
Suggested primer 2: | GGGTTGTAGATACCAGCCCA |