| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.058348 |
| Chromosome: | chromosome 2 |
| Location: | 6959471 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g120301 | SELH,SELENOH,SELH1 | (1 of 133) IPR012336 - Thioredoxin-like fold; Selenoprotein H | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGCTGCCCCACCGCCGCTGCTGCCGCTGCC |
| Internal bar code: | AGTATGATTATAGGACACCGCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 847 |
| LEAP-Seq percent confirming: | 98.8609 |
| LEAP-Seq n confirming: | 1215 |
| LEAP-Seq n nonconfirming: | 14 |
| LEAP-Seq n unique pos: | 21 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCTCCGCACATAGACCATTT |
| Suggested primer 2: | GGGTTGTAGATACCAGCCCA |