| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.058362 |
| Chromosome: | chromosome 1 |
| Location: | 96671 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g000650 | AMX2 | (1 of 2) 1.4.3.21 - Primary-amine oxidase / Copper amine oxidase; Copper amine oxidase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCGACGGGGCGCGGCCACACCCGAGTTGCA |
| Internal bar code: | GGCCCCCGGTTAGACGTCTGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 832 |
| LEAP-Seq percent confirming: | 99.6021 |
| LEAP-Seq n confirming: | 8761 |
| LEAP-Seq n nonconfirming: | 35 |
| LEAP-Seq n unique pos: | 54 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGCAACAATAGTTCCTCCCC |
| Suggested primer 2: | GCGTGTGTGTGTGTGTGTGT |