| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.058366 |
| Chromosome: | chromosome 15 |
| Location: | 192154 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre15.g635150 | (1 of 781) IPR000104 - Antifreeze protein, type I | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCAAACCCAAACCCGGATCCAGCACAGGCA |
| Internal bar code: | CAGCCAGACGATTCCCCAGAGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 326 |
| LEAP-Seq percent confirming: | 96.7901 |
| LEAP-Seq n confirming: | 392 |
| LEAP-Seq n nonconfirming: | 13 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACAAACCGGCCTTAAGAGA |
| Suggested primer 2: | AGGGAAAAGGACGAAGGAAA |