Insertion junction: LMJ.RY0402.058385_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.058385
Chromosome:chromosome 16
Location:5460742
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre16.g680950 (1 of 29) IPR029033 - Histidine phosphatase superfamily intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCCGCCGCCCCTGAAGGCCTTCACTGACGA
Internal bar code:TAGTTTACCTAACGCGCCTATA

Confirmation - LEAP-Seq

LEAP-Seq distance:776
LEAP-Seq percent confirming:99.5349
LEAP-Seq n confirming:214
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:AGGACCAACAATTCCAGTGC
Suggested primer 2:ACTGTCCACCAGACACTCCC