| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.058394 |
| Chromosome: | chromosome 12 |
| Location: | 6249416 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g536950 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGCAACCGCGAGCGGCGCCGTCGCGGCGAT |
| Internal bar code: | GGAGTGGTCTTTCAGGACGCAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 444 |
| LEAP-Seq percent confirming: | 99.3865 |
| LEAP-Seq n confirming: | 324 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATACAGGAGTGGGCCAACAG |
| Suggested primer 2: | GTAGACTGACGCTGGGGGTA |