| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.058402 |
| Chromosome: | chromosome 10 |
| Location: | 5520392 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g459300 | (1 of 78) IPR002110//IPR020683 - Ankyrin repeat // Ankyrin repeat-containing domain | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTGCGTCGTACGTGGCGGGAGAGCGCGCG |
| Internal bar code: | CATGTTAGTACTGTCGAGGTGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 862 |
| LEAP-Seq percent confirming: | 99.3719 |
| LEAP-Seq n confirming: | 2215 |
| LEAP-Seq n nonconfirming: | 14 |
| LEAP-Seq n unique pos: | 18 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGTACGCCAGCACCTATCA |
| Suggested primer 2: | GAACTCCAGTCCTCTGCCTG |