| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.058420 |
| Chromosome: | chromosome 8 |
| Location: | 5018589 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g386100 | CYP744A5P | 3'UTR_intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGAGACACAATGAAGGGGGCAGGGCATCTA |
| Internal bar code: | CAAATAGCGGCCGGGGCAGCAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 778 |
| LEAP-Seq percent confirming: | 98.1221 |
| LEAP-Seq n confirming: | 1672 |
| LEAP-Seq n nonconfirming: | 32 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAGGGTGATACTGCGTTTGG |
| Suggested primer 2: | TGCGGCTTCTTTGTTCTTTT |