| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.058421 |
| Chromosome: | chromosome_9 |
| Location: | 4489159 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre09.g395769 | FAP177 | sense | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | GAGGAAACGCGAACGATGTGGGACGCCGTA |
| Internal bar code: | CGGCCGCCACGCGACCTACCAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 587 |
| LEAP-Seq percent confirming: | 89.8773 |
| LEAP-Seq n confirming: | 1758 |
| LEAP-Seq n nonconfirming: | 198 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAAGAAGAACGTGGAGACGG |
| Suggested primer 2: | AAGAGTCCACATTCATGCCC |