| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.058465 |
| Chromosome: | chromosome 3 |
| Location: | 8166731 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g200400 | (1 of 1) PTHR30383 - PERIPLASMIC BROAD-SPECIFICITY ESTERASE/LIPASE/PROTEASE | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AATGAGGAATTTGCTGTCCGCTTGGGGCCC |
| Internal bar code: | CACGCGTTGCGCGCTCCCGGGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 836 |
| LEAP-Seq percent confirming: | 95.0555 |
| LEAP-Seq n confirming: | 2999 |
| LEAP-Seq n nonconfirming: | 156 |
| LEAP-Seq n unique pos: | 25 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGTGCATGGATCAGTGTGAC |
| Suggested primer 2: | AGTTCTGCTGTCTGCACCCT |